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  • Title: Beef-heart submitochondrial particles: a mixture of mitochondrial inner and outer membranes.
    Author: Albracht SP, Heidrich HG.
    Journal: Biochim Biophys Acta; 1975 Feb 17; 376(2):231-6. PubMed ID: 163647.
    Abstract:
    1. EPR spectra at 9 GHz and 83 degrees K of NADH-reduced anaerobic beef-heart submitochondrial particles, prepared from mitochondria by sonication and centrifugation, contain a signal (gz equals to 2.01, gy equals to 1.94, gx equals to 1.89) due to an iron-sulphur center of the mitochondrial outer membrane. 2. The ratio of inner and outer membranes in submitochondrial particles is not greatly different from that in beef-heart mitochondria. 3. Beef-heart submitochondrial particles free from outer-membrane contamination have been prepared by free-flow electrophoresis. EPR spectra at 83 degrees K of such particles are presented.
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