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  • Title: Target HCV NS3 CD4+ Th1 epitope to major histocompatibility complex class II pathway.
    Author: Gao M, Wang HP, Wang YN, Zhou Y, Wang QL.
    Journal: Biotechnol Lett; 2006 Jan; 28(1):3-8. PubMed ID: 16369867.
    Abstract:
    A hepatitis C virus (HCV) plasmid vaccine was constructed, based on class II-associated invariant chain peptide (CLIP) substitution which endogenously targets HCV non-structure protein 3 (NS3) CD4+ T helper 1(Th1) epitope (1248AA-1261AA) to major histocompatibility complex (MHC) class II antigen. The in vitro expression results demonstrated that the vaccine was expressed efficiently in COS-7 cell line. The expressed protein could co-localize in endo-membrane system with BALB/c mouse MHC class II molecule I-Ad. The recombinant invariant chain molecule could aggregate with BALB/c mouse I-Ad molecule and form the theoretical nonomer structure in the COS-7 cell line. The assembled molecules migrate to the cell surface by exocytosis. This has implications for HCV vaccine development.
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