These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Mast cell heterogeneity in various oral mucosal sites in the rat.
    Author: Matsson L.
    Journal: Arch Oral Biol; 1992; 37(6):445-50. PubMed ID: 1637259.
    Abstract:
    Two distinct types of mast cells are recognized in the rat: connective tissue mast cells (CTMCs), found in the peritoneal cavity, skin, tongue, etc. and mucosal mast cells (MMCs), found in the intestinal mucosa. The two subsets differ functionally and can be defined by histochemical methods. The aim here was to characterize the mast cell population in various oral mucosal sites. Biopsies were taken from the tongue, buccal mucosa, gingival mucosa and intestine (jejunum) of 20 rats. For optimal preservation of the MMCs, a fixative with low aldehyde concentration and low pH was used. The biopsies were embedded in paraffin. The first of three consecutive sections (5 microns) was stained with toluidine blue for 30 s, the second with toluidine blue for 7 days and the third with astra blue/safranine. Cells positive with toluidine blue after 30 s were classified as CTMCs, and those positive after 7 days but not after 30 s as MMCs. Cells positive to safranine in the astra blue/safranine staining sequence were classified as CTMCs and those positive to astra blue as MMCs. The total number of mast cells was similar in the superficial layers of all oral tissues studied. There were more mast cells in the deeper than in the superficial portions of the tongue. Mast cells with staining characteristics and size similar to those observed in the intestinal mucosa (MMCs) were found together with 'classical' connective tissue mast cells (CTMCs). The results suggest that the mast cell population of oral mucosal tissues of the rat contains both CTMC- and MMC-like cells.
    [Abstract] [Full Text] [Related] [New Search]