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  • Title: [Influence of sexual difference on expression of Toll-like receptor 4 and myeloid differential protein-2 mRNA in the lung in septic rats].
    Author: Du XH, Yao YM, Li R, Shen CA, Yin HN.
    Journal: Zhongguo Wei Zhong Bing Ji Jiu Yi Xue; 2005 Dec; 17(12):726-8. PubMed ID: 16386178.
    Abstract:
    OBJECTIVE: To investigate the influence of gender on the gene expression of Toll-like receptor 4 (TLR4), myeloid differential protein-2 (MD-2) and tumor necrosis factor-alpha (TNF-alpha) in the lung in septic rats. METHODS: The total RNA from normal and lipopolysaccharide (LPS)-challenged rats was extracted by Trizol reagent. The gene expressions of TLR4, MD-2 and TNF-alpha mRNA were measured by semi-quantitative reverse transcription polymerase chain reaction (RT-PCR). Plasma estradiol (E2) content was determined by radioimmunoassay. RESULTS: The gene of TLR4, MD-2 and TNF-alpha were expressed in the lungs in normal rats, and there was no significant difference between male and female rats (P>0.05). After LPS challenge, the gene expression were markedly up-regulated at 2 hours, and the gene expressions of TLR4, MD-2 and TNF-alpha were significantly lower in female group than those in male group (all P<0.01). CONCLUSION: There are marked difference in activation of TLR4 signal transduction in the lung when stimulated by LPS between male group and female group. The response to LPS is lower in female septic rats than in male septic rats, which might be associated to the effect of endogenous estrogen.
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