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  • Title: [Time-resolved fluoroimmunoassay of carcino-embryonic antigen and preparation of its diagnostic reagent].
    Author: Hang JF, Wu YS, Yu WH, Huang Y, Li M.
    Journal: Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi; 2006 Jan; 22(1):121-4. PubMed ID: 16388762.
    Abstract:
    AIM: To establish a two-site time-resolved fluoroimmunoassay (TRFIA) for detection of carcino-embryonic antigen(CEA) based on the direct sandwich technique(One monoclonal antibody(mAb) is immobilized on the surface of microtiter plate strip wells, another mAb is labelled with Eu(3+)) and prepare its diagnostic reagent. METHODS: The sandwich TRFIA for detection of serum CEA level using anti-CEA mAbs was used. RESULTS: The measure range of CEA-TRFIA was (1-560) microg/L. The analytical sensitivity was 0.28 mug/L. The intra- and inter-assay coefficients of variation (CV) were 7.2%-8.6% and 8.9%-13.2%, respectively. There was no cross-reaction to AFP, CA12-5, CA19-9 and albumin. Cross reactivity to CA15-3 was 1.62 microg/L. The correlation coefficient of blood samples detected by CEA-TRFIA and commercially available CEA CLIA (chemiluminescent immunoassay) kit (Roche) was 0.946. CONCLUSION: CEA-TRFIA is a new immunoassay which has several advantages including high sensitivity, specificity and a wide working range. It is suitable for routine clinical use.
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