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  • Title: [Screening of microorganisms--producers of alpha-L-rhamnosidase].
    Author: Rzaieva OM, Borzova NV, Varbanets' LD, Sokolova OV, Kharkevych OS, Zhdanova NM, Safronova LA.
    Journal: Mikrobiol Z; 2005; 67(5):19-27. PubMed ID: 16396108.
    Abstract:
    Screening of producers of alpha-L-rhamnosidase among 692 strains of microorganisms of different taxonomic groups has been performed. A capacity to synthesize the enzyme was revealed in 35.7% of the studied cultures. Representatives of genera Aspergillus, Myrothecium, Penicillium, Eupenicillium (activity 0.1 - 0.335 un./mg of protein) proved to be the most active producers. Complex preparations of alpha-L-rhamnosidase were obtained from cultural liquid of 10 producers by fractionation with ammonium sulfate (30 and 90% saturation); pH- and thermal optimum, as well as pH- and thermostability were analyzed in 7 of them. The Aspergillus, Penicillium, Eupenicillium strains which display high stability and activity under technological values of temperature 20-37 degrees C and medium pH 4.0-6.0 have been chosen for further investigations.
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