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  • Title: Isolation and characterization of alpha-glucosidase from Aspergillus niger.
    Author: Brízová K, Králová B, Demnerová K, Vins I.
    Journal: J Chromatogr; 1992 Feb 28; 593(1-2):125-31. PubMed ID: 1639895.
    Abstract:
    alpha-Glucosidase is an enzyme widely used in biochemical analytical methods. Aspergillus niger was selected as a potential source for its production. Conditions for glucosidase production were optimized and the enzyme was isolated from the culture supernatant by dialysis and anion-exchange chromatography. The activity of the enzyme was determined by maltose hydrolysis to glucose, which was determined using a glucose-specific electrode or by high-performance liquid chromatography. The isolated enzyme was further characterized by sodium dodecyl sulphate-polyacrylamide gel electrophoresis, substrate specificity and fast protein liquid chromatography. The Michaelis constant, optimal temperature and stability of the enzyme preparation were determined.
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