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  • Title: Determination of catecholamines in urine by liquid chromatography and electrochemical detection after on-line sample purification on immobilized boronic acid.
    Author: Eriksson BM, Wikström M.
    Journal: J Chromatogr; 1992 Feb 28; 593(1-2):185-90. PubMed ID: 1639903.
    Abstract:
    Norepinephrine, epinephrine and dopamine in urine were measured by an automated liquid chromatographic method. After sample purification on a column containing silica-immobilized boronic acid, which showed great affinity for catecholamines at neutral pH, the catecholamines were eluted by backflushing with an acidic mobile phase and transferred to a cation exchanger for separation. Detection was performed electrochemically and the relative standard deviation was 2% for the analysis of endogenous concentrations in human urine.
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