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  • Title: The efficiency of interference of Potato virus X infection depends on the target gene.
    Author: Takahashi S, Komatsu K, Kagiwada S, Ozeki J, Mori T, Hirata H, Yamaji Y, Ugaki M, Namba S.
    Journal: Virus Res; 2006 Mar; 116(1-2):214-7. PubMed ID: 16406166.
    Abstract:
    RNA silencing is a natural defense response against viral infection. This phenomenon has been used to interfere with viral infections by exploiting fragments of viral genomes as sources of RNA silencing. Agrobacterium-mediated transient expression of a hairpin RNA derived from the TGBp1 gene of Potato virus X (PVX) induced RNA silencing of the TGBp1 gene and resulted in interference of PVX infection. The interference was induced in the infiltrated leaves but not in the upper non-infiltrated leaves. Transient expression of a CP hairpin RNA also induced interference of PVX. The TGBp1 hairpin RNA showed more efficient interference of PVX infection than the CP hairpin RNA.
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