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  • Title: Interactions of the Escherichia coli hydrogenase biosynthetic proteins: HybG complex formation.
    Author: Butland G, Zhang JW, Yang W, Sheung A, Wong P, Greenblatt JF, Emili A, Zamble DB.
    Journal: FEBS Lett; 2006 Jan 23; 580(2):677-81. PubMed ID: 16412426.
    Abstract:
    Assembly of the active site of the [NiFe]-hydrogenase enzymes involves a multi-step pathway and the coordinated activity of many accessory proteins. To analyze complex formation between these factors in Escherichia coli, they were genomically tagged and native multi-protein complexes were isolated. This method validated multiple interactions reported in separate studies from several organisms and defined a new complex containing the putative chaperone HybG and the large subunit of hydrogenase 1 or 2. The complex also includes HypE and HypD, which interact with each other before joining the larger complex.
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