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  • Title: Effect of progesterone in vitro on luteinizing hormone production in hen pituitary cells pretreated with estrogen.
    Author: Kawashima M, Ukai A, Kamiyoshi M, Tanaka K.
    Journal: Poult Sci; 1992 Jul; 71(7):1215-20. PubMed ID: 1641386.
    Abstract:
    Isolated cells from the pituitary gland of nonlaying hens were preincubated in vitro with or without estradiol-17 beta (E2), and then incubated with or without progesterone (P4), and luteinizing hormone (LH) in the cells and media was measured by a homologous radioimmunoassay. When the cells were preincubated with E2 for 2 h and then incubated with P4 for 4 h, cellular LH was increased, but LH in the medium remained unchanged. The increase in cellular LH in response to P4 was dose-dependent. Protein synthesis inhibitors (cycloheximide, actinomycin-D, and alpha-amanitin) blocked the response of the cells to P4. The results suggest that LH production in estrogen-primed pituitary cells of the hen was stimulated by P4 through a protein-synthesizing pathway.
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