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  • Title: Fabrication of antibody arrays using thermally responsive elastin fusion proteins.
    Author: Gao D, McBean N, Schultz JS, Yan Y, Mulchandani A, Chen W.
    Journal: J Am Chem Soc; 2006 Jan 25; 128(3):676-7. PubMed ID: 16417330.
    Abstract:
    A general method has been developed to immobilize antibodies onto an array surface by employing fusion proteins consisting of an elastin domain with tunable hydrophobic properties and an antibody-binding domain with high binding affinity and specificity for antibodies. Antibodies conjugated with the elastin fusion proteins can be directly printed on a self-assembled monolayer-modified glass slide in a functionally active orientation with a spatially defined pattern. An antibody array sensor for detection of tumor markers was fabricated to demonstrate the utility of the method. We expect that the method presented here could be a simple and universal platform to immobilize antibodies for the fabrication of a variety of antibody array sensors.
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