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  • Title: The early stages of Saccharomyces cerevisiae yeast suspensions damage in moderate pulsed electric fields.
    Author: El Zakhem H, Lanoisellé JL, Lebovka NI, Nonus M, Vorobiev E.
    Journal: Colloids Surf B Biointerfaces; 2006 Feb 01; 47(2):189-97. PubMed ID: 16427256.
    Abstract:
    The objectives of this study were to investigate the effects of pulsed electric fields (PEF) application to colloidal suspension of Saccharomyces cerevisiae. The electrical conductivity measurements during the PEF-treatment of S. cerevisiae suspensions were used to monitor the extent of cell damages in the intervals of electric field strength E = 3-15 kV/cm and time of PEF treatment t(PEF) = 10(-4) to 1s. At relatively small fields (E < 7.5 kV/cm) the early stages of yeast cells damages were observed. At such treatment conditions, the damage was incomplete and developed at long time of PEF treatment, below the value of E = 7.5 kV/cm which is commonly referred in literature as a threshold for this culture. Data obtained for the disintegration in conductivity experiments were found in good correlation with direct counting of yeast lethality using light microscopy. The PEF-induced lethality of the yeast cells and size flocs increased with the mixing of suspensions and the increase of temperature.
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