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  • Title: New method for HPLC separation and fluorescence detection of malonaldehyde in normal human plasma.
    Author: Mao J, Zhang H, Luo J, Li L, Zhao R, Zhang R, Liu G.
    Journal: J Chromatogr B Analyt Technol Biomed Life Sci; 2006 Feb 17; 832(1):103-8. PubMed ID: 16439188.
    Abstract:
    A new method for the detection of free and total malonaldehyde (MDA) in human plasma samples based on the derivatization of MDA with 9-fluorenylmethoxycarbonyl hydrazine (FMOC-hydrazine) in an acidic medium was developed. Derivatization was achieved after 4 h at 50 degrees C. The derivatized samples were analyzed by HPLC using a reversed-phase C18 column with fluorescence detection (Ex=270 nm, Em=310 nm). The benefit of this direct injection of deproteinized plasma is to avoid the use of an internal standard. The detection limit was 0.1 pmol (4.0 nmol/L). The recovery of MDA spiked in different human plasma samples was 95.3% (n=25; R.S.D. 5.1%) for the hydrolysation procedure. The total and free MDA in plasma of 15 healthy male volunteers are 426+/-29.8 nmol/L and 153+/-9.6 nmol/L, respectively.
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