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  • Title: Detergent-resistant membranes are platforms for actinoporin pore-forming activity on intact cells.
    Author: Alegre-Cebollada J, Rodríguez-Crespo I, Gavilanes JG, del Pozo AM.
    Journal: FEBS J; 2006 Feb; 273(4):863-71. PubMed ID: 16441671.
    Abstract:
    Sticholysin II is a pore-forming toxin produced by the sea anemone Stichodactyla helianthus. We studied its cytolytic activity on COS-7 cells. Fluorescence spectroscopy and flow cytometry revealed that the toxin permeabilizes cells to propidium cations in a dose-dependent and time-dependent manner. This permeabilization is impaired by preincubation of cells with cyclodextrin. Isolation of detergent-resistant cellular membranes showed that sticholysin II colocalizes with caveolin-1 in fractions corresponding to raft-like domains. The interaction of sticholysin II with such domains is only lipid dependent as it also occurs in the absence of any other membrane-associated protein. Toxin binding to raft-like lipid vesicles inhibited cell permeabilization. The results suggest that sticholysin II promotes pore formation in COS-7 cells through interaction with membrane domains which behave like cellular rafts.
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