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  • Title: Cryo-EM and single particles.
    Author: Wang L, Sigworth FJ.
    Journal: Physiology (Bethesda); 2006 Feb; 21():13-8. PubMed ID: 16443818.
    Abstract:
    Cryoelectronmicroscopy is a method for the imaging of macromolecules in the electron microscope. It was originally developed to determine membrane protein structures from two-dimensional crystals, but more recently "single-particle" techniques have become powerful and popular. Three-dimensional reconstructions are obtained from sets of single-particle images by extensive computer processing; the methods are being applied to many macromolecular assemblies.
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