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  • Title: Non-viral heterogeneous sequences at the 5' ends of tomato spotted wilt virus mRNAs.
    Author: Kormelink R, van Poelwijk F, Peters D, Goldbach R.
    Journal: J Gen Virol; 1992 Aug; 73 ( Pt 8)():2125-8. PubMed ID: 1645150.
    Abstract:
    Subgenomic messenger RNAs transcribed from the tomato spotted wilt virus (TSWV) S RNA segment were partially purified from total RNA extracts of TSWV-infected Nicotiana rustica and analysed by primer extension analysis. The data obtained show the presence of non-viral sequences, 12 to 20 nucleotides in length, at the 5' ends of the N and NSs mRNAs, indicating a cap-snatching mechanism for the initiation of transcription. This is the first report of a plant virus using such a mechanism for transcription of the viral genome.
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