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  • Title: Inhibition of Na,K-ATPase by a new ATP analog, adenosine-5-N'-(2,4-dinitro-5-fluorophenyl)phosphohydrazide.
    Author: Lopina O, Skackova D, Baranova L, Khropov Y.
    Journal: FEBS Lett; 1991 May 06; 282(2):228-30. PubMed ID: 1645278.
    Abstract:
    A new ATP analog, adenosine-5-N'-(2,4-dinitro-5-fluorophenyl) phosphohydrazide (DNPH-AMP), has been synthesized, which is an irreversible inhibitor of Na,K-ATPase. Interaction of the analog with the enzyme in the presence of K+ is described by the scheme: [formula: see text] and corresponding kinetic constants k3 and Ki are found equal to 2.5 min-1 and 1.6 mM. In the presence of Na+ the time course of enzyme inactivation by DNPH-AMP is a biphasic curve in the semilogarithmic plot. The k3 and Ki values calculated for this case according to Fritzsch [Fritzsch (1985) J. Theor. Biol. 117, 397] are equal to 2.45 min-1 and 2.5 mM, respectively. ATP transforms the K(+)-type of Na,K-ATPase inactivation into the one that takes place in the presence of Na+.
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