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  • Title: 15N-excretion of heat-killed Lactobacillus casei in humans.
    Author: Wutzke KD, Sattinger V.
    Journal: Eur J Clin Nutr; 2006 Jul; 60(7):847-52. PubMed ID: 16452914.
    Abstract:
    OBJECTIVE: In the present study, Lactobacillus casei (L. casei) labelled with 15N was used to follow the metabolic fate of orally administered heat-killed 15N-labelled L. casei (15N-L.casei) in humans. DESIGN: Experimental study. SETTING: University of Rostock, Children's Hospital, Research Laboratory. SUBJECTS: Twelve healthy adults aged 23-32 years. INTERVENTION: The subjects received 36 mg/kg body weight heat-killed 15N-L.casei and 500 mg Lactose-[13C]ureide together with breakfast. Expired air samples were taken over 14 h, whereas urine and faeces were collected over 2 days. A blood sample was taken after 2 h. 13C- and 15N-enrichments were measured by isotope ratio mass spectrometry (SerCon, UK). RESULTS: The orocaecal transit time (OCTT) was reached after 4.1 h. The urinary 15N-excretion was 9.3% of the ingested dose, whereas the faecal excretion was 65.1% of the ingested dose. After 2 h, 15N-enrichment of supernatant, fibrinogen, and plasma protein precipitate amounted to 254, 11, and 2 p.p.m. excess, respectively. CONCLUSIONS: In comparison to the OCTT of 4.1 h, 15N-enrichment in urinary ammonia and urinary total nitrogen already began to rise 30 min after 15N-L.casei ingestion, indicating that 15N-L.casei is rapidly digested in the small bowel. This is confirmed by 15N-enrichments of blood plasma fractions. The ingestion of heat-killed 15N-L.casei led to a total excretion of 74.4% of the ingested 15N-dose.
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