These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Synthetic DNA bending sequences increase the rate of in vitro transcription initiation at the Escherichia coli lac promoter.
    Author: Gartenberg MR, Crothers DM.
    Journal: J Mol Biol; 1991 May 20; 219(2):217-30. PubMed ID: 1645411.
    Abstract:
    Appropriately phased DNA bending sequences replacing the CAP binding site upstream from the lac promoter increase by roughly tenfold the rate of specific transcription initiation from a superhelical promoter template in vitro; promoter occlusion results from polymerase binding to the upstream (dA)n.(dT)n tracts, but this phenomenon is not responsible for the observed phase-dependent transcriptional activity. The rates of open complex formation at both P1 and P2 promoters respond in a similar phase-dependent way to the synthetic curved DNA sequences.
    [Abstract] [Full Text] [Related] [New Search]