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  • Title: After microinjection hemimethylated DNA is converted into symmetrically methylated DNA before DNA replication.
    Author: Sandberg G, Guhl E, Graessmann M, Graessmann A.
    Journal: FEBS Lett; 1991 Jun 03; 283(2):247-50. PubMed ID: 1646121.
    Abstract:
    In this investigation we analysed the maintenance methylation activity of the mammalian cell DNA methyltransferase by microinjection of hemimethylated HSV-tk DNA into thymidine kinase-negative rat 2 cells. We found that the hemimethylated DNA was efficiently converted into symmetrical methylated molecules before DNA replication. Furthermore, integration of the trans-DNA into the host genome is an early event after gene transfer.
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