These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Resveratrol affects CYP1A expression in rainbow trout hepatocytes.
    Author: Aluru N, Vijayan MM.
    Journal: Aquat Toxicol; 2006 May 10; 77(3):291-7. PubMed ID: 16466815.
    Abstract:
    Resveratrol (RVT), a polyphenolic phytoalexin found in a variety of food products, including grapes and peanuts, is a well-characterized aryl hydrocarbon receptor (AhR) antagonist in mammalian cell lines. The lack of a reliable tool to block AhR signaling in rainbow trout (Oncorhynchus mykiss) prompted this study to evaluate the utility of RVT as an AhR antagonist in a piscine system. Trout hepatocytes in primary culture were exposed to varying doses of RVT (10(-5)-10(-12) M) either in the presence or absence of a well-established AhR agonist, beta-naphthoflavone (BNF). Indeed, BNF significantly elevated CYP1A and AhR protein expression in trout hepatocytes, and this response was inhibited at high (10(-5)-10(-7) M), but not low (10(-8)-10(-10) M), RVT doses. However, RVT alone at low doses (10(-8)-10(-10) M) significantly elevated CYP1A protein expression compared to control hepatocytes. This higher protein response with RVT was completely abolished with actinomycin D and cycloheximide suggesting transcriptional and translational regulation of CYP1A induction. Also, RVT at low (10(-9) M), but not high (10(-5) M), dose significantly elevated CYP1A1 mRNA transcript levels in BNF-primed hepatocytes supporting transcriptional modulation. Taken together, RVT is an effective AhR antagonist, but induces CYP1A expression at lower doses (<10(-8) M) in trout hepatocytes.
    [Abstract] [Full Text] [Related] [New Search]