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  • Title: Development and mathematical modeling of a two-stage reactor system for trichloroethylene degradation using Methylosinus trichosporium OB3b.
    Author: Hwang JW, Choi YB, Park S, Choi CY, Lee EY.
    Journal: Biodegradation; 2007 Feb; 18(1):91-101. PubMed ID: 16467965.
    Abstract:
    A two-stage reactor system was developed for the continuous degradation of gas-phase trichloroethylene (TCE). Methylosinus trichosporium OB3b was immobilized on activated carbon in a TCE degradation reactor, trickling biofilter (TBF). The TBF was coupled with a continuous stirred tank reactor (CSTR) to allow recirculation of microbial cells from/to the TBF for the reactivation of inactivated cells during TCE degradation. The mass transfer aspect of the TBF was analyzed, and mass transfer coefficient of 3.9 h(-1) was estimated. The loss of soluble methane monooxygenase (sMMO) activity was modeled based on a material balance on the CSTR and TBF, and transformation capacity (T (c)) was determined to be 20.2 micromol mg(-1). Maximum TCE degradation rate of 525 mg 1(-1) d(-1) was obtained and reactor has been stably operated for more than 270 days.
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