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  • Title: Expression of extracellular domain of human vascular endothelial growth factor receptor-2 in Pichia pastoris.
    Author: Zuo Q, Tian L, Hou JM, Wang YS, Wen YJ, Li J, Wei YQ.
    Journal: Sichuan Da Xue Xue Bao Yi Xue Ban; 2006 Jan; 37(1):1-4. PubMed ID: 16468629.
    Abstract:
    OBJECTIVE: To investigate the feasibility of high expressing extracellular domain of human vascular endothelial growth factor receptor-2 (heVEGFR-2) with eukaryotic protein structure in Pichia pastoris. METHODS: We used PCR to amplify the DNA fragment encoding heVEGFR-2 from pORF-heVEGFR-2. The recombinant Pichia pastoris secretory expression vector(pPICZalphaA-heVEGFR-2)was constructed and transferred into Pichia pastoris X-33 by electroporation. The high expression transformnants were identified through its drug-resistant phenotype and methanol induction. RESULTS: As indicated by SDS-PAGE, the recombinant heVEGFR-2 protein with a molecular weight (MW) approximately 108 kDa, which reached 80 mg/L in the mass concentration, comprised 45% of the total expressed secreted proteins from Pichia pastoris X-33. The section of heVEGFR-2 had a MW approximately 106 kDa. The results of western blot analysis demonstrated that this protein could be specifically recognized by the rat monoclonal antibody against mouse VEGFR-2 (rat McAb against mVEGFR-2). CONCLUSION: The heVEGFR-2 with eukaryotic protein structure can get a high expression in Pichia pastoris.
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