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  • Title: Production, isolation and characterization of human profilin from Saccharomyces cerevisiae.
    Author: Aspenström P, Lassing I, Karlsson R.
    Journal: J Muscle Res Cell Motil; 1991 Apr; 12(2):201-7. PubMed ID: 1648108.
    Abstract:
    The yeast Saccharomyces cerevisiae was used to express human profilin cDNA. The recombinant protein, isolated by affinity chromatography on poly(L-proline)-Sepharose followed by ion exchange chromatography, associates with non-muscle actin and phosphatidylinositol-(4,5)-bisphosphate as authentic profilin.
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