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  • Title: Indirect competitive immunoassay for detection of aflatoxin B1 in corn and nut products using the array biosensor.
    Author: Sapsford KE, Taitt CR, Fertig S, Moore MH, Lassman ME, Maragos CM, Shriver-Lake LC.
    Journal: Biosens Bioelectron; 2006 Jun 15; 21(12):2298-305. PubMed ID: 16495044.
    Abstract:
    Because of the potential health risks of aflatoxin B1 (AFB1), it is essential to monitor the level of this mycotoxin in a variety of foods. An indirect competitive immunoassay has been developed using the NRL array biosensor, offering rapid, sensitive detection and quantification of AFB1 in buffer, corn and nut products. AFB1-spiked foods were extracted with methanol and Cy5-anti-AFB1 added to the resulting sample. The extracted sample/antibody mix was passed over a waveguide surface patterned with immobilized AFB1. The resulting fluorescence signal decreased as the concentration of AFB1 in the sample increased. The limit of detection for AFB1 in buffer, 0.3 ng/ml, was found to increase to between 1.5 and 5.1 ng/g and 0.6 and 1.4 ng/g when measured in various corn and nut products, respectively.
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