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  • Title: A transfer RNA-dependent protein synthesizing system from Ehrlich ascites extracts.
    Author: Sharma OK, Roberts WK, Beezley DN, Borek E.
    Journal: Biochim Biophys Acta; 1975 May 16; 390(3):327-31. PubMed ID: 164951.
    Abstract:
    An homologous cell-free system from Ehrlich ascites cells was made tRNA dependent by passing both the ribosomes and the ammonium sulfate fraction through DEAE-cellulose columns in 0.30 M KCl. Protein synthesis was routinely stimulated 20-50 fold by the addition of 100 mug/ml of ascites cell tRNA in the presence of oviduct mRNA, ascites mRNA, or encephalomyocarditis RNA.
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