These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: [Determination of chloramphenicol residues in milk by reversed-phase high performance liquid chromatography with fluorescence detection].
    Author: Pan Y, Xu Q, Kang X, Zhang J.
    Journal: Se Pu; 2005 Nov; 23(6):577-80. PubMed ID: 16498984.
    Abstract:
    A high performance liquid chromatographic method with fluorescence detection for the determination of chloramphenicol (CAP) residues in milk was developed. Although CAP itself is non-fluorescent the aromatic nitro group of CAP could be reduced to aromatic primary amino group and fluorescamine can be used as a selective reagent for primary amines. Therefore, the CAP was derivatized with fluorescamine prior to injection in this project. The HPLC method was performed on a Diamond C18 column (250 mm x 4.6 mm i. d., 4.0 microm) with the mobile phase composed of sodium acetate buffer (0.02 mol/L, pH 6.0) -acetonitrile-tetrahydrofuran (76: 16: 8, v/v/v). The flow rate of the mobile phase was set at 1.0 mL/min, and the column was maintained at 40 degrees C. Analytes were detected by a fluorescence detector at 410 nm excitation and 508 nm emission wavelength. The standard curve was linear in the range from 0.4 microg/L to 800 microg/L. The limit of detection (LOD) was 0.2 microg/L, and the limit of quantitation (LOQ) was 0.4 microg/L. Overall recoveries were between 66.6% and 92.8% with relative standard deviations between 4.5% and 9.4%. The procedure provides a rapid, reliable and sensitive method for the determination of CAP in milk.
    [Abstract] [Full Text] [Related] [New Search]