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  • Title: Electrochemical detection of hepatitis B surface antigen using colloidal gold nanoparticles modified by a sol-gel network interface.
    Author: Tang D, Yuan R, Chai Y, Zhong X, Liu Y, Dai J.
    Journal: Clin Biochem; 2006 Mar; 39(3):309-14. PubMed ID: 16503333.
    Abstract:
    BACKGROUND: A novel potentiometric immunosensor for the detection of hepatitis B surface antigen has been developed by self-assembling gold nanoparticles to a thiol-containing sol-gel network. METHODS: A cleaned gold electrode was first immersed in a hydrolyzed (3-mercaptopropyl) trimethoxysilane sol-gel solution to assemble a three-dimensional silica gel, and then gold nanoparticles were absorbed onto the thiol groups of the sol-gel network. Finally, hepatitis B surface antibody was assembled onto the surface of the gold nanoparticles. The self-assembling procedure was characterized by cyclic voltammetry and electrochemical impedance spectroscopy. Detection is based on the change in potentiometric response before and after the antigen-antibody reaction. RESULTS: Tests relating to the detection of hepatitis B surface antigen demonstrate that the potentiometric immunosensor exhibited a rapid potentiometric response (<4 min), with high sensitivity, good reproducibility, and long-term stability. The linear range was from 4 to 960 ng.mL(-1) with a detection limit of 1.9 ng.mL(-1) (S/N = 3) and the lifetime was 1 month. CONCLUSION: Analytical results of several specimens using the developed technique showed satisfactory agreement with those from an ELISA method. This method shows promise for detecting HBsAg in clinical specimens.
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