These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: [Bioinformatics analysis of YZ-2 and preparation of polyclonal antibody].
    Author: Zang LQ, Qiu PX, Xiao R, Yan GM.
    Journal: Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi; 2006 Mar; 22(2):205-7. PubMed ID: 16507260.
    Abstract:
    AIM: To analyze YZ-2, a novel protein associated with cerebral ischemia and prepares its polyclonal antibody. METHODS: According to the bioinformatics analysis and prediction of the possible high structure, hydrophilicity and antigenicity of YZ-2, a 22-amino acid residue partial peptide of YZ-2 was synthesized. The synthesized peptide was then used to immunize. And the properties of anti-YZ2 were analyzed by ELISA and Western blot. RESULTS: The hydrophilicity and antigenicity were predicted by methods of bioinformatics. The polyclonal antibody of YZ-2 was successfully obtained and its specificity and sensitivity were conformed by ELISA and Western blot. CONCLUSION: By the bioinformatics analysis and prediction, the hydrophilicity and antigenicity of YZ2 were analyzed. The antibody of YZ-2 was successfully obtained.
    [Abstract] [Full Text] [Related] [New Search]