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  • Title: Crystallization and preliminary X-ray characterization of a lectin from Cicer arietinum (chickpea).
    Author: Katre UV, Gaikwad SM, Bhagyawant SS, Deshpande UD, Khan MI, Suresh CG.
    Journal: Acta Crystallogr Sect F Struct Biol Cryst Commun; 2005 Jan 01; 61(Pt 1):141-3. PubMed ID: 16508116.
    Abstract:
    The lectin isolated from mature seeds of Cicer arietinum (CAL) agglutinates pronase-treated rabbit and human erythrocytes and its haemagglutination activity is inhibited by fetuin and desialated fetuin but not by simple monosaccharides or oligosaccharides. The purified lectin is a dimer of molecular weight 43,000 Da composed of two identical subunits (MW 21,500), as confirmed by SDS-PAGE. The lectin has been crystallized using the hanging-drop vapour-diffusion method at 295 K over a well solution containing 0.2 M sodium acetate, 0.1 M sodium phosphate buffer pH 6.5 and 14%(w/v) polyethylene glycol 8000. The triangular prism-shaped crystals belong to space group R3 and have unit-cell parameters a = b = 81.2, c = 69.4 A. The diffraction data are 93.8% complete to 2.3 A Bragg spacing with an Rmerge of 0.103.
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