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  • Title: Compact soft x-ray transmission microscopy with sub-50 nm spatial resolution.
    Author: Kim KW, Kwon Y, Nam KY, Lim JH, Kim KG, Chon KS, Kim BH, Kim DE, Kim J, Ahn BN, Shin HJ, Rah S, Kim KH, Chae JS, Gweon DG, Kang DW, Kang SH, Min JY, Choi KS, Yoon SE, Kim EA, Namba Y, Yoon KH.
    Journal: Phys Med Biol; 2006 Mar 21; 51(6):N99-107. PubMed ID: 16510949.
    Abstract:
    In this paper, the development of compact transmission soft x-ray microscopy (XM) with sub-50 nm spatial resolution for biomedical applications is described. The compact transmission soft x-ray microscope operates at lambda = 2.88 nm (430 eV) and is based on a tabletop regenerative x-ray source in combination with a tandem ellipsoidal condenser mirror for sample illumination, an objective micro zone plate and a thinned back-illuminated charge coupled device to record an x-ray image. The new, compact x-ray microscope system requires the fabrication of proper x-ray optical devices in order to obtain high-quality images. For an application-oriented microscope, the alignment procedure is fully automated via computer control through a graphic user interface. In imaging studies using our compact XM system, a gold mesh image was obtained with 45 nm resolution at x580 magnification and 1 min exposure. Images of a biological sample (Coscinodiscus oculoides) were recorded.
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