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  • Title: Crystallization and preliminary X-ray diffraction analysis of a chitin-binding domain of hyperthermophilic chitinase from Pyrococcus furiosus.
    Author: Nakamura T, Ishikawa K, Hagihara Y, Oku T, Nakagawa A, Inoue T, Ataka M, Uegaki K.
    Journal: Acta Crystallogr Sect F Struct Biol Cryst Commun; 2005 May 01; 61(Pt 5):476-8. PubMed ID: 16511072.
    Abstract:
    The crystallization and preliminary X-ray diffraction analysis of the chitin-binding domain of chitinase from a hyperthermophilic archaeon, Pyrococcus furiosus, are reported. The recombinant protein was prepared using an Escherichia coli overexpression system and was crystallized by the hanging-drop vapour-diffusion method. An X-ray diffraction data set was collected to 1.70 A resolution. The crystal belonged to space group P4(3)2(1)2 or P4(1)2(1)2. The unit-cell parameters were determined to be a = b = 48.8, c = 85.0 A.
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