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  • Title: Purification, partial characterization and preliminary X-ray diffraction analysis of a mannose-specific lectin from Cymbosema roseum seeds.
    Author: Cavada BS, Marinho ES, Souza EP, Benevides RG, Delatorre P, Souza LA, Nascimento KS, Sampaio AH, Moreno FB, Rustiguel JK, Canduri F, de Azevedo WF, Debray H.
    Journal: Acta Crystallogr Sect F Struct Biol Cryst Commun; 2006 Mar 01; 62(Pt 3):235-7. PubMed ID: 16511310.
    Abstract:
    A lectin from Cymbosema roseum seeds (CRL) was purified, characterized and crystallized. The best crystals grew in a month and were obtained by the vapour-diffusion method using a precipitant solution consisting of 0.1 M Tris-HCl pH 7.8, 8%(w/v) PEG 3350 and 0.2 M proline at a constant temperature of 293 K. A data set was collected to 1.77 A resolution at a synchrotron-radiation source. CRL crystals are orthorhombic, belonging to space group P2(1)2(1)2(1). Crystallographic refinement and full amino-acid sequence determination are in progress.
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