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  • Title: [Inhibitory effect of cholecystokinin octapeptide on lipopolysaccharide-elicited inducible nitric oxide synthase expression in vascular endothelial cells].
    Author: Yan J, Gu ZY, Wang XY, Liu S, Xu XH, Cong B, Ling YL.
    Journal: Zhongguo Wei Zhong Bing Ji Jiu Yi Xue; 2006 Feb; 18(2):96-100. PubMed ID: 16512640.
    Abstract:
    OBJECTIVE: To investigate the effect of cholecystokinin octapeptide (CCK-8) on lipopolysaccharide (LPS)-elicited inducible nitric oxide synthase (iNOS) expression in vascular endothelial cells. METHODS: Human umbilical vein endothelial cell line (ECV-304 cells) was stimulated with vehicle (normal saline) or LPS in the presence (0.01, 0.1, 1 mg/L) or absence (0.1 mg/L) of CCK-8 (10(-6)-10(-8)mol/L). Nitric oxide (NO) level and cellular nitric oxide synthase (NOS) activity were determined with spectrophotometrically. The iNOS expression was detected with immunocytochemical technique and Western blot. RESULTS: Compared with normal saline, LPS significantly induced the upregulation of iNOS protein expression in the cultured ECV-304 cells, and NOS activity in ECV-304 cells and NO level in cultured media were increased. CCK-8 obviously inhibited above-mentioned effect of LPS in a dose-dependent manner. Whereas CCK-8 alone did not showed effect on iNOS protein expression, NO level and cellular NOS activity as compared with those values when vehicle was used. CONCLUSION: CCK-8 inhibited LPS-elicited iNOS expression and NO production in ECV-304 cells.
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