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  • Title: Novobiocin-dependent topA deletion mutants of Escherichia coli.
    Author: Hammond GG, Cassidy PJ, Overbye KM.
    Journal: J Bacteriol; 1991 Sep; 173(17):5564-7. PubMed ID: 1653212.
    Abstract:
    Previous reports of the transduction of topA deletions in Escherichia coli suggested that delta top A transductants grow normally only if they acquire spontaneous mutations that compensate for the topoisomerase I defect. We show that P1-mediated transduction of delta topA in the presence of sublethal concentrations of novobiocin, an inhibitor of the DNA gyrase B subunit, yields uncompensated Top- isolates which are dependent on novobiocin for optimum growth. In the absence of novobiocin these delta topA strains grow slowly, indicating that topA deletions are deleterious but not lethal to the cell. We propose that inhibitors of DNA gyrase B, presumably by lowering intracellular levels of DNA supercoiling, can phenotypically suppress a topoisomerase I defect in E. coli.
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