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  • Title: Identification of possible artifacts in the association of official analytical chemists sporicidal test.
    Author: Miner NA, Mulberry GK, Starks AN, Powers-Prather A, Entrup M, Armstrong M, Maida B.
    Journal: Appl Environ Microbiol; 1995 Apr; 61(4):1658-60. PubMed ID: 16535009.
    Abstract:
    Two laboratories tested four different brands of alkaline 2% glutaraldehyde sterilants by the Association of Official Analytical Chemists sporicidal test. Each laboratory found survival of Clostridium sporogenes spores on spore-labeled unglazed porcelain penicylinders (cylinders) to vary from test to test, and survival did not always correlate with increasing sterilant exposure time. These results were consistent with a theory that there may be random conditions within the test that prevent the sterilant from contacting all spores. Further studies indicated that the prior history of the unglazed porcelain cylinders and whether the C. sporogenes culture grown in egg-meat media had been processed (homogenized) to eliminate visible pieces of egg-meat media were important factors affecting the results and repeatability of this test.
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