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  • Title: Modified quantitative association of official analytical chemists sporicidal test for liquid chemical germicides.
    Author: Miner N, Armstrong M, Carr CD, Maida B, Schlotfeld L.
    Journal: Appl Environ Microbiol; 1997 Aug; 63(8):3304-7. PubMed ID: 16535679.
    Abstract:
    The Association of Official Analytical Chemists (AOAC) test for sporicidal activity of disinfectants (966.04) is used in the United States as the legal criteria for classifying a liquid as a chemical sterilant and also as the indicator of the highest level of disinfectant. This qualitative test contains procedures that may cause inaccurate results. A modified, quantitative version of the AOAC sporicidal test has been developed that uses a specified minimum number of spores (2.0 x 10(sup5)) of Bacillus subtilis or Clostridium sporogenes dried onto porcelain penicylinders. This modified test was validated with three commercial sterilant chemicals tested on three separate groups of spore-labeled cylinders.
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