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  • Title: Reduction of cytochrome C by ascorbic free radical.
    Author: Paciolla C, De Gara L.
    Journal: Boll Soc Ital Biol Sper; 1991 Feb; 67(2):137-44. PubMed ID: 1653579.
    Abstract:
    The rate of "in vivo" reduction of cytochrome c by ascorbic acid (AA) increases from 69 nmoles of cytochrome c for minute, to 202 nanomoles when ascorbate oxidase is added. Since the AA oxidation by AA oxidase is a system to generate ascorbic free radical (AFR), data suggest that AFR is a better reducing compound than ascorbate in cytochrome c reduction. Since the addition of oxidized glutathione and human immunoglobulins (-S-S- bridge containing compounds) in the medium produces a remarkable decrease in cytochrome c reduction, it is suggested that AFR could also reduce -S-S- groups.
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