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  • Title: A ribonuclease from the wild mushroom Boletus griseus.
    Author: Wang H, Ng TB.
    Journal: Appl Microbiol Biotechnol; 2006 Oct; 72(5):912-6. PubMed ID: 16544139.
    Abstract:
    A ribonuclease (RNase) with a molecular mass of 29 kDa and cospecific for poly A and poly U was isolated from fruiting bodies of the mushroom Boletus griseus. Its N-terminal sequence exhibited some similarity to those of RNases from the mushrooms Irpex lacteus and Lentinus edodes. The RNase was adsorbed on diethylaminoethyl-cellulose, Q-Sepharose, and Affi-gel blue gel and was unadsorbed on CM-cellulose. The enzyme exhibited a temperature optimum between 60 and 70 degrees C and a pH optimum at 3.5.
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