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  • Title: A rapid procedure for the purification of cardiac 1,4-dihydropyridine receptors from porcine heart.
    Author: Haase H, Striessnig J, Holtzhauer M, Vetter R, Glossmann H.
    Journal: Eur J Pharmacol; 1991 May 25; 207(1):51-9. PubMed ID: 1655494.
    Abstract:
    Highly purified porcine cardiac sarcolemma was used as a source for purification of mammalian cardiac 1,4-dihydropyridine receptors associated with the voltage-dependent Ca2+ channel. The cardiac digitonin-solubilized receptor prelabeled with (+)-[3H]PN 200-110 was enriched at least 236-fold using an improved, rapid three-step purification protocol which could be completed within 12 h. The purity of the preparation was at least 22%, the yield of the receptors 24%. Photoaffinity labeling experiments with (-)-[3H]azidopine allowed the identification of the cardiac alpha 1 subunit. In contrast to the purified rabbit or guinea-pig skeletal muscle Ca2+ channel complex, none of the purified polypeptides underwent rapid and substantial phosphorylation by the catalytic subunit of the cyclic AMP-dependent protein kinase in vitro.
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