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  • Title: Mapping of rfa Genes in Salmonella typhimurium by ES18 and P22 Transduction and by Conjugation.
    Author: Kuo TT, Stocker BA.
    Journal: J Bacteriol; 1972 Oct; 112(1):48-57. PubMed ID: 16559160.
    Abstract:
    Loci termed rfa, determining biosynthesis of somatic lipopolysaccharide core, have been mapped in Salmonella typhimurium LT2. The smooth-specific phage P22 co-transduced two leaky rfa alleles with cysE and with pyrE; one of the leaky alleles is perhaps rfaG, and the other is an unidentified gene concerned with synthesis of the heptose-containing part of the core. The lipopolysaccharide-indifferent phage ES18 (or its variant ES18.h1) co-transduced rfaF, rfaG, rfaL, rfa(R-res-1), and rfa(R-res-2) alleles with cysE and with pyrE, at rates indicating the order cysE-rfaF-(rfa[R-res-1], rfa[r-res-2], rfaL)-rfaG-pyrE. One proven (and two suspected) rfaE alleles and five proven rfaH alleles were not co-transduced with cysE or pyrE. Hfr crosses indicated that the proven rfaE allele mapped between serA and strA.
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