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  • Title: [Composite matrix membranes as synthetic receptor systems. 2. Structural-morphological characteristics].
    Author: Kochkodan VM, Honcharuk VV, Hilal N.
    Journal: Ukr Biokhim Zh (1999); 2005; 77(4):64-9. PubMed ID: 16568605.
    Abstract:
    Structural and morphological characteristics of composite imprinted membranes for selective recognizing of adenosine 3',5'-cyclic monophosphate (cAMP) were studied. Composite polyvinylidene fluoride microfiltration membranes (Millipore) covered with a thin imprinted polymer layer were prepared using photoinitiated copolymerization of dimethylaminoethylmetacrylate with trimethylolpropanethrimethacrylate in the presence of cAMP as template. Atomic force microscopy (AFM) and scanning electron microscopy (SEM) were used to visualise surfaces and cross-sections of imprinted membranes and to determine their structural and morphological parameters such as pore size, thickness of selective imprinted layer, surface roughness as well as total surface contact area. The impact of structural characteristics on separation properties of the imprinted membranes was analyzed. It was found out that the thickness of the imprinted polymer layer with optimal recognizing properties is limited.
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