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  • Title: [Preparation of soybean isoflavone aglycones by reversed-phase high performance liquid chromatography from Semen Sojae Praeparatum].
    Author: Qu L, Mi H, Fan G.
    Journal: Zhong Yao Cai; 2005 Dec; 28(12):1062-3. PubMed ID: 16568661.
    Abstract:
    OBJECTIVE: An efficient method for the isolation of isoflavone aglycones from Semen Sojae Praeparatum was developed. METHODS: The crude isoflavones were extracted by 75% aqueous ethanol and primarily purified by 1300 macroporous resin,and then the fractions containing daidzein, glycitein and genistein were obtained and subjected to preparative HPLC for the isolation of target components. RESULTS: Daidzein, glycitein and genistein were obtained with purities of over 99%, etermined by analytical HPLC. CONCLUSION: This technique is simple and suitable for the isolation of isoflavone aglycones from Semen Sojae Praeparatum.
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