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  • Title: Metal-enhanced fluorescence-based RNA sensing.
    Author: Aslan K, Huang J, Wilson GM, Geddes CD.
    Journal: J Am Chem Soc; 2006 Apr 05; 128(13):4206-7. PubMed ID: 16568977.
    Abstract:
    In this communication, we present the proof-of-principle of a new RNA sensing technique, based on metal-enhanced fluorescence, which may improve the potential of solid substrate-based RNA capture assays. In this regard, the detection of RNA is accomplished by annealing a target RNA tagged with a fluorophore to a DNA anchor probe in a single step on a solid surface, where the fluorescence signal is enhanced by silver nanoparticles. Furthermore, the proposed method is highly amenable to high-throughput screening applications and is capable of detecting less than 25 fmol of RNA at a signal-to-noise ratio greater than 20, in several hours compared to that of reverse transcription-PCR at >24 h.
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