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  • Title: Nuclear localization of budgerigar fledgling disease virus capsid protein VP2 is conferred by residues 308-317.
    Author: Rihs HP, Peters R, Hobom G.
    Journal: FEBS Lett; 1991 Oct 07; 291(1):6-8. PubMed ID: 1657638.
    Abstract:
    The capsid protein VP2 of budgerigar fledgling disease virus (BFDV) contains two sequences (residues 309-315 and 334-340) which are homologous to the prototypic nuclear localization sequence (NLS) of the simian virus 40 T-antigen. Using recombinant potential NLS-beta-galactosidase fusion proteins we identified amino acid residues 308-317 (VPKRKRKLPT) to be the NLS of BFDV capsid proteins VP2 and VP3. Microfluorometry studies show that the BFDV-VP2 signal is considerably more efficient in nuclear transport kinetics, than the NLS of SV40-VP2, corresponding to amino acid residues 317-326 (PNKKKRKLSR).
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