These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: The hygromycin B-resistance-encoding gene as a selectable marker for stable transformation of Trypanosoma brucei.
    Author: Lee MG, van der Ploeg LH.
    Journal: Gene; 1991 Sep 15; 105(2):255-7. PubMed ID: 1657720.
    Abstract:
    The hygromycin B (Hy) phosphotransferase-encoding gene (hph), was tested as a selectable marker in the protozoan, Trypanosoma brucei. The hph gene was placed under the control of the promoter of a procyclic acidic repetitive protein-encoding gene, and was integrated by homologous recombination into an intergenic region of the alpha beta-tubulin-encoding gene tandem array of T. brucei. In contrast to many other selectable markers tested, spontaneous Hy resistance was not observed, making Hy a second useful marker for transformation of this protozoan.
    [Abstract] [Full Text] [Related] [New Search]