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  • Title: Cloning and functional expression of a novel endoprotease involved in prohormone processing at dibasic sites.
    Author: Nakayama K, Hosaka M, Hatsuzawa K, Murakami K.
    Journal: J Biochem; 1991 Jun; 109(6):803-6. PubMed ID: 1657897.
    Abstract:
    We cloned and sequenced a cDNA from a library of mouse pituitary AtT-20 cells which are known to cleave an endogenous and various foreign prohormones at dibasic sites. This cDNA encodes a novel 753-residue protein, named PC3, which is structurally related to the yeast Kex2 protease involved in precursor cleavage at dibasic sites and to recently identified mammalian Kex2-like proteins, furin and PC2. Among examined cell lines and tissues, PC3 mRNA was only detected in AtT-20 cells. The substrate specificity of PC3 expressed in mammalian cells was similar to that observed in AtT-20 cells. We conclude that PC3 is a resident prohormone processing endoprotease in AtT-20 cells.
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