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  • Title: Addition of glycerol for improved methylation linkage analysis of polysaccharides.
    Author: Kim JS, Reuhs BL, Michon F, Kaiser RE, Arumugham RG.
    Journal: Carbohydr Res; 2006 Jun 12; 341(8):1061-4. PubMed ID: 16580651.
    Abstract:
    A presolubilization procedure with the use of glycerol is shown to be applicable for the structural analysis of polysaccharides. Neutral, acidic, high-molecular-weight and low-molecular-weight polysaccharides were solubilized in glycerol prior to methylation and subsequent linkage analysis by GC-MS. All four types of polysaccharides showed significant increases in derivatization following presolubilization as measured by recovery of partially methylated alditol acetates.
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