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  • Title: Identification of a cloned cytokinin biosynthetic gene.
    Author: Barry GF, Rogers SG, Fraley RT, Brand L.
    Journal: Proc Natl Acad Sci U S A; 1984 Aug; 81(15):4776-80. PubMed ID: 16593495.
    Abstract:
    A small region of the Ti plasmid (the tmr locus), thought to be involved in phytohormone metabolism in Agrobacterium tumefaciens-transformed plant tissue, was cloned and expressed in Escherichia coli. By enzyme assay, the tmr locus was shown to encode isopentenyltransferase, an enzyme that catalyzes the first step in cytokinin biosynthesis.
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